Vampires and werewolves have waged a nocturnal war against each other for centuries. But all bets are off when a female vampire warrior named Selene, who's famous for her strength and werewolf-hunting prowess, becomes smitten with a peace-loving male werewolf, Michael, who wants to end the war.

As the war between the vampires and the Lycans rages on, Selene, a former member of the Death Dealers (an elite vampire special forces unit that hunts werewolves), and Michael, the werewolf hybrid, work together in an effort to unlock the secrets of their respective bloodlines.

David Huxley, low-born self-made legal counsel at the US embassy in Budapest, may expect his career to be boosted by his impending marriage to Tish, daughter of old money tycoon and ambassador to Hungary Harrington. But when the happy couple spends a night of hot sex at a spa, including a threesome with an exhibitionist model, they are secretly filmed, and the suspect is found murdered in their hotel. To handle the blackmail, they venture into a sex club where porno movies are made. Tish volunteers to 'act' so David can snoop around, but that only gets them into even greater danger, which ultimately proves most deceptive.

Picking up directly from the previous movie, vampire warrior Selene and the half werewolf Michael hunt for clues to reveal the history of their races and the war between them.

Selene, a vampire warrior, is entrenched in a conflict between vampires and werewolves, while falling in love with Michael, a human who is sought by werewolves for unknown reasons.